Question

Que tipo de placa de ensaio usar em experimentos de luminescência, fluorescência ou multiplex?

Answer 1

Em síntese:

• Luminescência: placa branca
• Fluorescência: placa preta
• Multiplex: de acordo com a leitura que se pretende otimizar (branca para aumentar a sensibilidade do parâmetro que emprega luminescência; preta para reduzir ruído para o parâmetro baseado em leitura de fluorescência) 

Detalhamento:

Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. The clear bottom plates offer the advantage of being able to examine the cells by microscopy during the course of the experiment. The primary difference between white and black plates is their reflective properties. White plates reflect light and will maximize light output signal; black plates absorb light and reduce background and crosstalk. For these reasons, white plates are commonly used for luminescent assays and black plates are used for fluorescent assays. When multiplexing a luminescent and fluorescent assay, the use of a white plate would support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for fluorescence. The use of a black plate in a multiplex assay would reduce fluorescent signal crosstalk and background with a reduction in luminescence

Fonte:  Promega, artigo "Which plates should I choose for fluorescence and luminescence measurements?" (acessado em 16/Fev/2017)

Answer 2

Síntese de conclusões reproduzidas de matéria "How to choose the best microplate colour for my assay", da BMG LabTech:

1. For absorbance assays you will need wells with clear bottoms
2. For absorbance-based measurements below 320 nm, you must use UV-transparent plates (e.g., cycloolefin microplates)
3. Black plates are recommended for fluorescence measurements. They significantly reduce commonly encountered autofluorescence and high background signals
4. For luminescence assays, white plates are recommended to amplify the signal since luminescence assays typically generate weak signals and have low background. 
5. Grey plates additionally reduce cross-talk. For particularly bright luminescence assays, a black plate is also recommended.