IEC - Perguntas recentes com a tag fluorescência

Quais as características do Vetor Amaxa pmaxGFP?

Wed, 31 May 2023 11:40:20 +0000

Reagente: Carboxynaphthofuorescein diacetate

Mon, 21 Aug 2017 22:05:13 +0000

5(6)-Carboxynaphthofluorescein (CBNF; CAS 163831-68-3) reagent, recommended as a live cell dye to use with the Agilent Cell Kit (Cat# 5067-1519). Product discontinued (Thermo Cat# C13196), but recommended in your document "Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer, Agilent Technologies Application Note, (2001), publication #5988-4320EN."

Molecular Probes #C13196 (descontinuado) - Carboxynaphthofluorescein diacetate (CNFDA) (C13196, ), which is cleaved by intracellular esterases to yield red-fluorescent carboxynaphthofluorescein (excitation/emission maxima ~598/668 nm), is the only long-wavelength tracer of this type that can be passively loaded into live cells. Like chloromethyl SNARF-1 acetate, carboxynaphthofluorescein diacetate is usually used in combination with a green-fluorescent tracer for detecting cell–cell interactions.[1]. Setareh Biotech Catalog ID: 6962 - Fluorogenic substrate for esterases which is cleaved by intracellular esterases to yield red-fluorescent

Molecular Probes #C653 5-(and-6)-Carboxynaphthofluorescein, Succinimidyl Ester, mixed isomers
Naphthofluorescein has emission maxima of approximately 670 nm in aqueous solution at pH 10. The fluorescence from this dye is pH dependent (pKa ~7.6) requiring a relatively alkaline pH for maximal fluorescence. T The amine reactive 5-(and-6)-carboxynaphthofluorescein succinimidyl ester can be used to couple this fluorophore to biomolecules of interest.
Setareh Biotech Catalog ID: 6964: Amine-Reactive fluorescent probe for peptide or protein conjugation

Espectro: vide este link

(Molecular probes) Handbook Ch-15 - Assays for Cell Viability, Proliferation and Function

Quais as propriedades da ZsGreen1 GFP?

Thu, 09 Mar 2017 20:18:15 +0000

Informações do site da Takara Clontech (vide https://goo.gl/auFJkZ):

ZsGreen1 Fluorescent Protein
Living Colors ZsGreen1 is a bright green fluorescent protein derived from a Zoanthus sp. reef coral (1) that has been modified for high solubility, bright emission, and rapid chromophore maturation. The ZsGreen1 fluorescent protein has been reported to have better expression than EGFP in lung and muscle tissue and in cultured cells (2).
Features
Exceptional brightness: 2.5 times brighter than EGFP
ZsGreen1 excitation and emission maxima are 493 and 505 nm, respectively
References
Matz, M. V. et al. (1999) Nature Biotechnol. 17(10):969–973. Erratum in: Nature Biotechnol. (1999) 17(12):1227. Fluorescent proteins from nonbioluminescent Anthozoa species.
Bell, P. et al. (2007) J. Histochem. Cytochem. 55(9):931–939. A comparative analysis of novel fluorescent proteins as reporters for gene transfer studies.

Que tipo de placa de ensaio usar em experimentos de luminescência, fluorescência ou multiplex?

Thu, 16 Feb 2017 15:11:22 +0000