IEC - Perguntas recentes com a tag cultura-de-células

Que tipo de microplaca devo usar para meu experimento?

Tue, 01 Aug 2023 19:06:05 +0000

Como efetuar ensaio para análise de migração e invasão celular in vitro?

Fri, 02 Mar 2018 13:35:40 +0000

Vote nas sugestões que foram mais úteis!

Informações básicas sobre cultura celular

Fri, 02 Mar 2018 13:30:57 +0000

Reagente: Carboxynaphthofuorescein diacetate

Mon, 21 Aug 2017 22:05:13 +0000

5(6)-Carboxynaphthofluorescein (CBNF; CAS 163831-68-3) reagent, recommended as a live cell dye to use with the Agilent Cell Kit (Cat# 5067-1519). Product discontinued (Thermo Cat# C13196), but recommended in your document "Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer, Agilent Technologies Application Note, (2001), publication #5988-4320EN."

Molecular Probes #C13196 (descontinuado) - Carboxynaphthofluorescein diacetate (CNFDA) (C13196, ), which is cleaved by intracellular esterases to yield red-fluorescent carboxynaphthofluorescein (excitation/emission maxima ~598/668 nm), is the only long-wavelength tracer of this type that can be passively loaded into live cells. Like chloromethyl SNARF-1 acetate, carboxynaphthofluorescein diacetate is usually used in combination with a green-fluorescent tracer for detecting cell–cell interactions.[1]. Setareh Biotech Catalog ID: 6962 - Fluorogenic substrate for esterases which is cleaved by intracellular esterases to yield red-fluorescent

Molecular Probes #C653 5-(and-6)-Carboxynaphthofluorescein, Succinimidyl Ester, mixed isomers
Naphthofluorescein has emission maxima of approximately 670 nm in aqueous solution at pH 10. The fluorescence from this dye is pH dependent (pKa ~7.6) requiring a relatively alkaline pH for maximal fluorescence. T The amine reactive 5-(and-6)-carboxynaphthofluorescein succinimidyl ester can be used to couple this fluorophore to biomolecules of interest.
Setareh Biotech Catalog ID: 6964: Amine-Reactive fluorescent probe for peptide or protein conjugation

Espectro: vide este link

(Molecular probes) Handbook Ch-15 - Assays for Cell Viability, Proliferation and Function

Como utilizar os pipetadores automáticos Biohit e Pipetman Concept

Thu, 01 Jun 2017 11:45:20 +0000

Qual o protocolo de detecção de contaminação por micoplasma vigente no laboratório?

Fri, 05 May 2017 15:54:17 +0000

Dados sobre células HEK293?

Thu, 16 Feb 2017 19:48:51 +0000

Dados sobre células NP69?

Thu, 16 Feb 2017 19:34:13 +0000

Ondo encontro informações sobre os fundamentos dos ensaios para análise de viabilidade, citotoxicidade e morte celular?

Thu, 16 Feb 2017 19:21:04 +0000

Como efetuar avaliação de clonogênese e transformação celular in vitro?

Thu, 16 Feb 2017 13:03:55 +0000