Como verificar a edição genômica com tecnologia CRISPR/Cas9?

Fri, 19 Feb 2021 12:46:28 +0000

Quais as propriedades da ZsGreen1 GFP?

Thu, 09 Mar 2017 20:18:15 +0000

Informações do site da Takara Clontech (vide https://goo.gl/auFJkZ):

ZsGreen1 Fluorescent Protein
Living Colors ZsGreen1 is a bright green fluorescent protein derived from a Zoanthus sp. reef coral (1) that has been modified for high solubility, bright emission, and rapid chromophore maturation. The ZsGreen1 fluorescent protein has been reported to have better expression than EGFP in lung and muscle tissue and in cultured cells (2).
Features
Exceptional brightness: 2.5 times brighter than EGFP
ZsGreen1 excitation and emission maxima are 493 and 505 nm, respectively
References
Matz, M. V. et al. (1999) Nature Biotechnol. 17(10):969–973. Erratum in: Nature Biotechnol. (1999) 17(12):1227. Fluorescent proteins from nonbioluminescent Anthozoa species.
Bell, P. et al. (2007) J. Histochem. Cytochem. 55(9):931–939. A comparative analysis of novel fluorescent proteins as reporters for gene transfer studies.

Quais as principais estratégias para manipulação gênica e clonagem molecular?

Fri, 17 Feb 2017 22:20:06 +0000

IEC - Perguntas recentes em Manipulação genética

Como verificar a edição genômica com tecnologia CRISPR/Cas9?

Quais as propriedades da ZsGreen1 GFP?

ZsGreen1 Fluorescent Protein

Features

References

Quais as principais estratégias para manipulação gênica e clonagem molecular?