IEC - Perguntas e respostas recentes em Instrucional

Respondida: Como preparar uma ficha catalográfica para produções acadêmicas?

Sat, 22 Jan 2022 12:02:45 +0000

Pode ser utilizada ferramenta disponibilizada pelo setor de bibliotecas da UNESP, em https://www.biblioteca.unesp.br/ficha/).

Nesse caso, recomendável colocar abaixo da ficha:

"*Gerada pelo autor com auxílio parcial do sistema de geração de fichas catalográficas da UNESP (https://www.biblioteca.unesp.br/ficha/)."

Respondida: Que softwares alternativos ao FlowJo podem ser usados para análises de citometria de fluxo?

Mon, 05 Apr 2021 21:09:51 +0000

As indicações abaixo são de postagem em https://listoffreeware.com/free-flowing-software-windows/

Uma cópia em PDF da publicação está disponível neste link

5 Best Free Flowing Software For Windows
(...)
My Favorite Free Flowing Software For Windows:
Flowing Software is my favorite flow cytometry software for Windows. It provides a good number of tools to analyze FCS files. Plus, its user interface is quite simple and intuitive.
You may also like PID Simulator Software, Data Visualization Tools, and Statistical Analysis Software for Windows.
Flowing Software
Flowing Software is a simple and easy to use flow cytometry software for Windows. It comes with various tools to analyze data from flow cytometry experiments. It lets you perform flow cytometry analysis on data stored in FCS and TXT (tab delimited) files. It provides a wide range of tools in different categories which are as follows:
Visualization Tools: This category contains main graphical analysis functions including Dot Plot, Density Plot, Intensity Plot, Histogram, and Overlay Histogram.
Metadata Tools: Here, you can find Metadata Viewer, Compensation Matrix, Parameter Data Viewer, and Keyword Viewer.
Calculation Tools: Statistics Calculator, Calculate Ratio of Two Parameters, Advanced Cell Calculator, and Token tools can be accessed from here.
Parameter Modification Tools: From here, you can change parameter names, change lin/log visualization, and create time axis.
Data Modification Tools: You can find Virtual Gain and Compensate Data functions here.
Collection Tools: This category offers you tools like Image Collector, Collector List, etc.
Other Tools: Some additional tools like Text Box, Tool Group, Region Control Tools, Automation Tools (Folder Runner, Update Counter, etc.), etc. are also available in this flowing software.
How to perform flow cytometry analysis using Flowing Software
Firstly, from File menu, open New Analysis. Now, from Create menu, select a tool for flow cytometry analysis. You can now open a FCS or TXT file containing flow cytometry experiments and analyse its related data. It lets you select multiple tools one by one and open related analysis on the main interface.
You can create statistics (events, mean, geomean, media, etc.), create regions, copy data to clipboard export data to TXT file, etc.
You can save the entire analysis in its native format for later use.
All in all, this one is a great flowing software to use to carry flow cytometry analysis. It is quite easy to use but in case you find any difficulty, you can refer to this video tutorial.
Home Page | Download Page
FlowPy
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Cyflogic
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FCSalyzer
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Cellular Symphony
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Komal Srivastava
Technical Content Editor

Respondida: Como devem ser descritos e documentados experimentos de qPCR?

Fri, 29 Mar 2019 13:05:03 +0000

Para experimentos de qPCR existem requisitos mínimos para descrição e documentação, indicados no artigo abaixo:

Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 55(4):611-22, 2009. [PMID: 19246619]

Vide também informações e um checklist útil em http://www.rdml.org/miqe.php

Respondida: Como desenhar iniciadores (primers) para experimentos baseados em PCR?

Thu, 14 Mar 2019 13:43:25 +0000

Vide apresentação "Considerações acerca de oligonucleotídeos utilizados em experimentos de PCR", disponível em https://virican.net/lnk/primers

Respondida: Qual o significado dos símbolos usados para indicação de riscos e cuidados requeridos no ambiente laboratorial?

Wed, 13 Mar 2019 15:07:18 +0000

Vide artigo "Science Laboratory Safety Symbols and Hazard Signs, Meanings" do Labmanager.com, disponível em https://goo.gl/nhaJ2d.

Versão em PDF disponível no LabDocs, também acessível via https://goo.gl/HjBhyg

Respondida: Quais são os tipos mais comuns de graus de pureza para reagentes laboratoriais?

Wed, 13 Mar 2019 14:46:44 +0000

Artigo informativo obtido de Labmanager.com (acesso via https://goo.gl/o5zHPN):

The Seven Most Common Grades for Chemicals and Reagents
It is imperative that everyone in the custody supply chain know and understand the different grades of chemicals and their uses
By Aaron Schieving | November 13, 2017
Chemicals and reagents play a critical role in the manufacturing and testing of pharmaceutical products, medical devices, biologics, cell- and tissue-based products, and many other healthcare-related solutions. Laboratories and researchers who use chemicals and reagents trust that their manufacturers have properly identified the grades of each chemical and ensured that the chemicals have met all regulatory and compliance standards for their intended use. It is imperative that everyone in the custody supply chain know and understand the different grades of chemicals and their uses, which are explained in this article.
When making a solution, the manufacturer must first decide what degree of chemical purity is needed based on the intended use. The following list describes the seven most common grades for chemicals and reagents, from highest to lowest grade/purity:
ACS grade meets or exceeds purity standards set by the American Chemical Society (ACS). This grade is acceptable for food, drug, or medicinal use and can be used for ACS applications or for general procedures that require stringent quality specifications and a purity of ≥95%.

Reagent grade is generally equal to ACS grade (≥95%) and is acceptable for food, drug, or medicinal use and is suitable for use in many laboratory and analytical applications.

USP grade meets or exceeds requirements of the United States Pharmacopeia (USP). This grade is acceptable for food, drug, or medicinal use. It is also used for most laboratory purposes, but the USP being followed should always be reviewed prior to beginning to ensure the grade is appropriate for that methodology.

NF grade meets or exceeds requirements of the National Formulary (NF). The USP and the NF (USP– NF) jointly publish a book of public pharmacopeial standards for chemical and biological drug substances, dosage forms, compounded preparations, excipients, medical devices, and dietary supplements. The listings here should be reviewed to determine which would be considered equivalent grades.

Laboratory grade is the most popular grade for use in educational applications, but its exact levels of impurities are unknown. While excellent for teaching and training, it is not pure enough to be offered for food, drug, or medicinal use of any kind.

Purified grade, also called pure or practical grade, meets no official standard; it is not pure enough to be offered for food, drug, or medicinal use of any kind.

Technical grade is used for commercial and industrial purposes; however, like many others, it is not pure enough to be offered for food, drug, or medicinal use of any kind.
ACS, Reagent, and USP-NF grades are typically equivalent and interchangeable but, even so, appropriateness should always be confirmed before application. This can be done by reviewing the applicable regulatory requirements.
Lab, purified, and technical grades have their own uses. For example, lab-grade chemicals, because of their low cost and good chemical purity, are used widely in educational applications, such as teaching laboratories at both the secondary school and college levels; however, lab-grade chemicals would not be appropriate for use in the quality control laboratory of a pharmaceutical or medical device manufacturer. ACS-, USP-, or reagent-grade chemicals should be applied in this setting, because they have fewer impurities that could ultimately impact patients taking the drugs made with those chemicals.
With seven different and inequivalent types of chemical purity grades, it is crucial to understand how they can impact products. Using a lower-purity grade than a product’s intended use requires could be a costly mistake. Similarly, using a higher-purity grade when not required could result in unnecessary costs. Add in the increased regulatory scrutiny and it becomes even more important to have a complete understanding of the components that your process requires.

Respondida: Onde encontro online esquemas e figuras úteis sobre cânceres, carcinogênese, etc?

Fri, 23 Nov 2018 12:40:06 +0000

Cell Snapshots: acesse seção específica sobre cânceres em https://goo.gl/7HeiLE

Temas disponíveis em 23/11/2018:
Acute Myeloid Leukemia, Bladder Cancer, Bone Metastasis, Breast Cancer, Cancer Metabolism Pathways, Cancer Vaccines, Cancer Vaccines, Chronic Lymphocytic Leukemia, Diffuse Large B Cell Lymphoma, The Epithelial-Mesenchymal Transition, Fanconi Anemia and Associated Proteins, Genetic Models of Cancer, Glioblastoma Multiforme, Hepatocellular Carcinoma, Immune Checkpoint Inhibitors, Lung Cancer Models, Medulloblastoma, Melanoma, MicroRNAs in Cancer, Multiple Myeloma, Non-Small Cell Lung Cancer, Pancreatic Cancer, Prostate Cancer, Renal Cell Carcinoma, Renal Cell Carcinoma, TCGA-Analyzed Tumors, Tumor Angiogenesis.

Respondida: Onde encontro informações oficiais sobre classificação de vírus?

Thu, 16 Feb 2017 19:15:33 +0000

Vide página de taxonomia viral do ICTV (International Committee on Taxonomy of Viruses)