As estratégias mais comuns explicadas em uma série de artigos da IDT DNA:
- Cloning strategies, Part 1: Assembly PCR for novel gene synthesis - Learn how you can use single-stranded oligos or a mix of single- and double-stranded DNA to produce longer genes of up to several thousand base pairs. No restriction sites are needed, and the approach is beneficial for assembling constructs that contain modular elements, such as antibodies.
- Cloning strategies, Part 2: Cohesive-end cloning - Cohesive-end cloning is one of the most commonly employed techniques in molecular biology. Review these tips and tricks for cloning using restriction enzymes.
- Cloning strategies, Part 3: Blunt-end cloning - Cloning of double-stranded DNA into plasmid vectors is one of the most commonly employed techniques in molecular biology. The procedure is used for sequencing, building libraries of DNA molecules, expressing coding and non-coding RNA, and many other applications. Learn about blunt-end cloning as a third method by which DNA fragments can be cloned into a plasmid vector.
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